Processes and systems for preparation of nucleic acid sequencing libraries and libraries prepared using same

Number of patents in Portfolio can not be more than 2000

United States of America Patent

PATENT NO 10221436
APP PUB NO 20160257984A1
SERIAL NO

14990276

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Abstract

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This disclosure provides methods for preparing a sequencing library including the steps of providing a template nucleic acid sequence, dNTPs, dUTP, a primer, a polymerase, a dUTP excising enzyme, and a plurality of beads including oligonucleotide adapter sequence segments; amplifying the template nucleic acid with the polymerase, dNTPs, dUTP and random hexamer to provide a complementary nucleic acid sequence including occasional dUTPs; and excising the incorporated dUTPs with the dUTP excising enzyme to provide nicks in the complementary nucleic acid sequence to provide a sequencing library.

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Patent Owner(s)

Patent OwnerAddress
10X GENOMICS INC6230 STONERIDGE MALL ROAD PLEASANTON CA 94588-3260

International Classification(s)

Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Belhocine, Kamila Fremont, US 30 270
Bjornson, Keith Fremont, US 41 1231
Hardenbol, Paul San Francisco, US 68 2689
Hindson, Benjamin Pleasanton, US 86 3417
Patel, Pranav Fremont, US 44 429
Wu, Indira San Carlos, US 11 53
Wyatt, Paul William Pleasanton, US 2 52

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