Molecular identity tags and uses thereof in identifying intermolecular ligation products

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United States of America Patent

PATENT NO 10385334
APP PUB NO 20160108394A1
SERIAL NO

14894653

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Abstract

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Molecular identity tags and methods of marking target nucleic acids with molecular identity tags for identifying intermolecular ligation products. Terminal regions of linear target nucleic acids are marked with pairs of distinguishable molecular identity tags. Each linear target nucleic acid is marked with only one distinguishable pair. The terminal ends of the marked target nucleic acids are joined to generate circularized nucleic acids, thereby juxtaposing two molecular identity tags across the joined terminal ends. Circularized nucleic acids or downstream products thereof comprising juxtaposed unpaired molecular identity tags constitute intermolecular nucleic acid products that can be identified and eliminated from subsequent analyses of the nucleic acids. The molecular identity tags may comprise physically linked and non-physically linked pairs. Physically linked molecular identity tags designed for production and analysis of mate-pair libraries with next-generation sequencing platforms are provided.

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Patent Owner(s)

Patent OwnerAddress
LOK SIHONGKONG

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Lok, Si Hong Kong, CN 58 328

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