Methods for identifying T-cell receptor antigens
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United States of America
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Jan 7, 2020
Grant Date -
Nov 14, 2019
app pub date -
Apr 23, 2019
filing date -
Apr 23, 2019
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Abstract
The present disclosure automated methods for creating cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of antigens that bind to T-cell receptors. The engineered peptides are preferably expressed in the cells under conditions that provide both secretion and display of the engineered peptides on the cell surfaces, thus providing access of the engineered peptide antigens to identify potential TCR binding targets. The cell libraries cab be engineered using an automated editing system that provides for one or more targeted edits per cell.
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