Processes and systems for preparation of nucleic acid sequencing libraries and libraries prepared using same

Number of patents in Portfolio can not be more than 2000

United States of America

PATENT NO 10557158
APP PUB NO 20190169666A1
SERIAL NO

16228362

Stats

ATTORNEY / AGENT: (SPONSORED)

Importance

Loading Importance Indicators... loading....

Abstract

See full text

This disclosure provides methods for preparing a sequencing library including the steps of providing a template nucleic acid sequence, dNTPs, dUTP, a primer, a polymerase, a dUTP excising enzyme, and a plurality of beads including oligonucleotide adapter sequence segments; amplifying the template nucleic acid with the polymerase, dNTPs, dUTP and random hexamer to provide a complementary nucleic acid sequence including occasional dUTPs; and excising the incorporated dUTPs with the dUTP excising enzyme to provide nicks in the complementary nucleic acid sequence to provide a sequencing library.

Loading the Abstract Image... loading....

First Claim

See full text

Family

Loading Family data... loading....

Patent Owner(s)

Patent OwnerAddress
10X GENOMICS INC6230 STONERIDGE MALL ROAD PLEASANTON CA 94588-3260

International Classification(s)

Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Belhocine, Kamila Fremont, US 30 270
Bjornson, Keith Fremont, US 41 1231
Hardenbol, Paul San Francisco, US 68 2689
Hindson, Benjamin Pleasanton, US 86 3417
Patel, Pranav Fremont, US 44 429
Wu, Indira San Carlos, US 11 53
Wyatt, Paul William Pleasanton, US 2 52

Cited Art Landscape

Load Citation

Patent Citation Ranking

Forward Cite Landscape

Load Citation

Maintenance Fees

Fee Large entity fee small entity fee micro entity fee due date
3.5 Year Payment $1600.00 $800.00 $400.00 Aug 11, 2023
7.5 Year Payment $3600.00 $1800.00 $900.00 Aug 11, 2027
11.5 Year Payment $7400.00 $3700.00 $1850.00 Aug 11, 2031
Fee Large entity fee small entity fee micro entity fee
Surcharge - 3.5 year - Late payment within 6 months $160.00 $80.00 $40.00
Surcharge - 7.5 year - Late payment within 6 months $160.00 $80.00 $40.00
Surcharge - 11.5 year - Late payment within 6 months $160.00 $80.00 $40.00
Surcharge after expiration - Late payment is unavoidable $700.00 $350.00 $175.00
Surcharge after expiration - Late payment is unintentional $1,640.00 $820.00 $410.00