Processes and systems for preparation of nucleic acid sequencing libraries and libraries prepared using same
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United States of America
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Feb 11, 2020
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Jun 6, 2019
app pub date -
Dec 20, 2018
filing date -
Dec 20, 2018
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Abstract
This disclosure provides methods for preparing a sequencing library including the steps of providing a template nucleic acid sequence, dNTPs, dUTP, a primer, a polymerase, a dUTP excising enzyme, and a plurality of beads including oligonucleotide adapter sequence segments; amplifying the template nucleic acid with the polymerase, dNTPs, dUTP and random hexamer to provide a complementary nucleic acid sequence including occasional dUTPs; and excising the incorporated dUTPs with the dUTP excising enzyme to provide nicks in the complementary nucleic acid sequence to provide a sequencing library.
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