Copy number preserving RNA analysis method

Number of patents in Portfolio can not be more than 2000

United States of America

PATENT NO 10597699
APP PUB NO 20180171384A1
SERIAL NO

15127672

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Abstract

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The present invention provides a method for generating an amplified nucleic acid portion of a template RNA molecule, comprising after having obtained a template RNA, annealing a first oligonucleotide primer at a preselected 3′ terminal nucleic acid region of the template RNA, elongating the first oligonucleotide primer in a template specific manner thereby obtaining a first elongated strand, removing the RNA template, annealing one or more further oligonucleotide primers to the first elongated strand, elongating the one or more further oligonucleotide primers in a template specific manner without strand displacement of polynucleotides annealed to the first elongated strand or using a polymerase that destroys a displaced strand, thereby generating further elongation products, isolating and/or amplifying an elongation product of said further elongation product comprising a nucleic acid portion that is elongated complementary to the first oligonucleotide primer; as well as kits for performing the method.

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Patent Owner(s)

Patent OwnerAddress
LEXOGEN GMBH1030 VIENNA

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Moll, Pamela Vienna, AT 3 0

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