Library construction using Y-adapters and vanishing restriction sites

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United States of America Patent

PATENT NO 10689690
APP PUB NO 20170044600A1
SERIAL NO

15235236

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Abstract

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The present disclosure describes systems and methods to construct genomic DNA fragments library by stretching genomic DNA over an adapter-modified chip; using an enzyme cocktail comprising a first restriction enzyme, a ligase, and a second restriction enzyme to cut the genomic DNA into fragments and ligate the fragments to the adapters on the chip; and amplifying thus-obtained DNA by PCR amplification.

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Patent Owner(s)

Patent OwnerAddress
CENTRILLION TECHNOLOGY HOLDINGS CORPORATIONGRAND CAYMAN KY1-1104

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Costa, Justin Palo Alto, US 22 44

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