System for continuous mutagenesis in vivo to facilitate directed evolution

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United States of America Patent

PATENT NO 10760071
APP PUB NO 20180216097A1
SERIAL NO

15747282

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Abstract

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A system for continuous mutagenesis to facilitate directed evolution, the system including DNA polymerases carrying the novel K54E point mutation, and other point mutations including I709N, A759R, D424A (herein called K54E_LF Pol I) and this methods of use to produce and detect lines where mutagenesis is continuous and does not exhibit the usual decline in mutagenesis with sequential cloning.

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Patent Owner(s)

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THE REGENTS OF THE UNIVERSITY OF CALIFORNIA1111 FRANKLIN STREET 12TH FLOOR OAKLAND CA 94607

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Camps, Manel Santa Cruz, US 6 5

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