Method for obtaining 1-kestose

Number of patents in Portfolio can not be more than 2000

United States of America

PATENT NO 10982246
APP PUB NO 20190085364A1
SERIAL NO

16166215

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Abstract

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The present invention discloses an industrial scale method to obtain 1-kestose by the use of a recombinant fructosyltransferase (FTF), isolated from Festuca arundinacea, expressed constitutively in a non-saccharolytic yeast. In this invention, the recombinant FTF type sucrose:sucrose 1-fructosyltransferase (1-SSTrec) is produced constitutively, stable and at high yield, both in the culture supernatant and in intact cells of the host Pichia pastoris. Hence, the invention additionally provides a method for 1-SST production at industrial scale. The recombinant enzyme is then used for mass production of short-chain fructooligosaccharides (FOS), specifically 1-kestose, from sucrose. The method of the present invention establishes conditions that allow conversion rates where the synthesized FOS constitute above 55% (w/w) of the total sugars in the reaction mixture and the 1-kestose content reaches values higher than 90% (w/w) of the total FOS fraction.

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Patent Owner(s)

Patent OwnerAddress
CENTRO DE INGENIERIA GENETICA Y BIOTECNOLOGIACIUDAD DE LA HABANA 10600

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Feijoo, Costa Gumersindo Ames, ES 2 0
Hernández, Garcia Lázaro Havana, CU 1 0
Lema, Rodicio Juan Manuel Teo, ES 3 9
Martínez, García Duniesky Sancti Spíritus, CU 2 0
Menéndez, Rodríguez Carmen Havana, CU 2 0
Pérez, Cruz Enrique Rosendo Sancti Spíritus, CU 2 0
Ramírez, Ibañez Ricardo Havana, CU 2 0
Sobrino, Legón Alina Sancti Spíritus, CU 2 0
Trujillo, Toledo Luis Enrique Havana, CU 2 0

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