Formulations and process for isolating viable microorganisms from positive blood cultures

Number of patents in Portfolio can not be more than 2000

United States of America

PATENT NO 11225681
APP PUB NO 20200087702A1
SERIAL NO

16677092

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Abstract

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Various embodiments disclosed herein provide for reagents and methods for rapidly isolating viable microbial cells, including S. pneumoniae, from positive blood culture samples. The resulting microbial pellet can be used for both identification and growth-based methods such as antimicrobial susceptibility testing. The buffers described herein may contain a base solution, non-ionic detergents, thiols, and optionally, ammonium chloride. The disclosed methods provide a process for rapidly isolating and concentrating viable microorganism(s) from PBC samples using only one sample preparation tube and centrifugation while removing cellular debris from the mammalian blood cells that may interfere with identification methods.

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Patent Owner(s)

Patent OwnerAddress
BECTON DICKINSON AND COMPANY1 BECTON DRIVE MC110 FRANKLIN LAKES NJ 07417-1880

International Classification(s)

Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Beaty, Patrick Shawn Dallastown, US 13 90
Brasso, William B Columbia, US 10 53
Bruton, Jeffery H Randallstown, US 2 1
Callihan, Donald R Cockeysville, US 3 3
Douglass, John P York, US 2 1
Feng, Liping Baltimore, US 15 72
Gosnell, Curtis M Fallston, US 4 2
Kircher, Susan M Hanover, US 9 53
Luper, Dyan Cockeysville, US 3 49
Malick, Adrien P Granite, US 11 334
Mantlo, John D Sykesville, US 14 9
Rosales, Julie L Randallstown, US 6 18
Turng, Ben Tucson, US 13 155
White, Vanda Baltimore, US 7 52
Zhou, James Y Columbia, US 3 1

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