Methods and hybrids for targeted nucleic acid editing in plants using CRISPR/Cas systems

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United States of America Patent

PATENT NO 11492630
APP PUB NO 20180223295A1
SERIAL NO

15575167

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Abstract

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The present invention relates to methods and hybrids for the targeted modification of a nucleic acid-target region in a plant target structure using CRISPR/Cas systems. The invention specifically relates to methods and hybrids for directly obtaining a plant or plant material which comprises an editing of a nucleic acid introduced in a targeted manner into a meristematic cell. The hybrids can be introduced in a transient and/or stable manner. The invention also relates to novel plant-optimized introduction strategies. The invention further relates to a method for carrying out an in vitro screening assay in order to first check the suitable gRNA candidates in vitro with respect to their efficiency.

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Patent Owner(s)

Patent OwnerAddress
KWS SAAT SE & CO KGAAGRIMSEHLSTR 31 EINBECK 37574

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Harling, Hinrich Bovenden, DE 4 22
Jongedijk, Erik Lokeren, BE 12 44
Martin-Ortigosa, Susana Einbeck, DE 8 53
Niessen, Markus Hannover, DE 17 63
Schumann, Nadine Einbeck, DE 3 7
Streitner, Corinna Melle, DE 5 7

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