Avoiding epigenetic silencing of exogenous nucleic acid in algae
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United States of America Patent
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Feb 14, 2023
Grant Date -
Jun 18, 2020
app pub date -
Dec 12, 2019
filing date -
Dec 13, 2018
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Abstract
The present application relates to the identification of novel DNA methyltransferases including CHG methylation in algal species. The present application relates to algal mutants permitting the expression of exogenous genes by alleviating the epigenetic mechanisms of CHG and CHH methylation of exogenous DNA and mono- and tri-methylation of lysine 9 of histone 3 (H3K9). This is achieved by mutating or attenuating the methyltransferase (MTase) genes in algae. The present application also relates to methods for efficiently expressing exogenous genes in algal species.
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