Generation of new varieties of Cannabis by chemical mutagenesis of cannabis cell suspensions
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United States of America Patent
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Apr 11, 2023
Grant Date -
Apr 9, 2020
app pub date -
Oct 7, 2019
filing date -
Oct 5, 2018
priority date (Note) -
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Abstract
An method of generating and selecting mutant Cannabis plants through mutagenesis of isolated Cannabis plant cells includes subjecting plant parts of one or more Cannabis plants to a pectinase treatment to obtain living cells of the one or more Cannabis plants, suspending the living cells in a mutagenic solution comprising methane sulfonate (EMS) and dimethyl sulfoxide (DMSO) to obtain mutated Cannabis cells, centrifuging the mutated Cannabis cells to obtain pelleted cells, and providing the pelleted cells on culture media.
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