Visual continuous spatial directed evolution method

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United States of America Patent

PATENT NO 11702764
APP PUB NO 20200332282A1
SERIAL NO

16904549

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Abstract

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A visual continuous spatial directed evolution method is disclosed. The host grows and moves in a solid culture space, the host carrying a foreign target gene to be evolved and containing a gene element that assists the evolution of the target gene, the target gene being correlated with the growth and movement of the host. Depending on different spatial distribution patterns formed in the solid culture space during the growth and movement of the host, screening is performed to obtain an evolved product. This method is carried out directly in the solid culture space. Depending on images of different spatial distribution morphologies visible to the naked eye that are locally formed, selection of evolved products is performed without the need for liquid fed-batch culture equipment. In addition, the evolution effect is visually observed through the infection spots formed during evolution, so that no real-time monitoring equipment is required.

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Patent Owner(s)

Patent OwnerAddress
SHENZHEN INSTITUTES OF ADVANCED TECHNOLOGY1068 XUEYUAN AVENUE XILI UNIVERSITY TOWN NANSHAN DISTRICT SHENZHEN GUANGDONG 518055

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Chen, Qian Shenzhen, CN 416 1749
Lai, Wangsheng Shenzhen, CN 2 0
Liu, Chenli Shenzhen, CN 6 0
Sun, Chenjian Shenzhen, CN 2 0
Wei, Ting Shenzhen, CN 13 63

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