Multiplex genome engineering in eukaryotes

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United States of America Patent

PATENT NO 11725204
APP PUB NO 20210222155A1
SERIAL NO

16097091

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Compositions and methods for gene editing are provided. The methods employ an oligo-based annealing mechanism that is rooted in the process of DNA replication rather than homologous recombination (HR). Oligo incorporation efficiencies are comparable and often exceed those of CRISPR/cas9 editing without the need for double strand breaks (DSBs). By relying on the multiplex annealing of oligos rather than DSBs the process is highly scalable across a genomic region of interest and can generate many scarless modifications of a chromosome simultaneously. Combinatorial genomic diversity can be generated across a population of cells in a single transformation event; genomic landscapes can be traversed through successive iterations of the process, and genome-wide changes can be massively parallelized and amplified through systematic strain mating.

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Patent OwnerAddress
YALE UNIVERSITYTWO WHITNEY AVENUE NEW HAVEN CT 06510

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Barbieri, Edward Lindenhurst, US 2 0
Isaacs, Farren Stamford, US 7 2

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