Methods for the improvement of product yield and production in a microorganism through glycerol recycling

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United States of America Patent

PATENT NO 11753656
APP PUB NO 20160194669A1
SERIAL NO

14911102

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Abstract

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The present invention provides for novel metabolic pathways to reduce or modulate glycerol production and increase product formation. More specifically, the invention provides for a recombinant microorganism comprising one or more native and/or heterologous proteins that function to import glycerol and one or more native and/or heterologous enzymes that function in one or more engineered metabolic pathways to convert a carbohydrate source, such as lignocellulose, to a product, such as ethanol, wherein the one or more native and/or heterologous proteins or enzymes is activated, upregulated, or downregulated. The invention also provides for a recombinant microorganism comprising one or more native or heterologous proteins that function to regulate glycerol synthesis and one or more native and/or heterologous enzymes that function in one or more engineered metabolic pathways to convert a carbohydrate source to ethanol, wherein said one or more native and/or heterologous proteins or enzymes is activated, upregulated or downregulated. Also provided are methods for increasing cellular glycerol uptake and increasing recombinant production of fuels and other chemicals using the recombinant microorganisms of the invention.

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Patent Owner(s)

Patent OwnerAddress
DANSTAR FERMENT AGSWISS EXPRESS ZUG ZUG

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Argyros, Aaron Etna, US 62 157
Kenealy, William R Carlsbad, US 7 16
Stonehouse, Emily Etna, US 12 66

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