Mammalian cell line for protein production and library generation

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United States of America Patent

PATENT NO 11802281
APP PUB NO 20200325468A1
SERIAL NO

16090829

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Abstract

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According to a first aspect of the invention, a method for the generation of a cell line is provided, comprising the steps of (a) providing a plurality of mammalian B cells, wherein each of the plurality of B cells comprises a transgenic genomic DNA sequence encoding a marker protein inserted into an endogenous immunoglobulin locus comprised in said B cell, and wherein the transgenic genomic DNA sequence is amenable to cleavage by a site directed nuclease, particularly Cas9; (b) replacing the transgenic genomic DNA sequence encoding a marker protein with a second transgenic DNA sequence encoding a protein of interest; (c) sorting B cells based on the presence or absence of the marker protein; and (d) collecting B cells in which the marker protein is absent.

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Patent Owner(s)

Patent OwnerAddress
ETH ZURICH8092 ZURICH

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Kelton, William Basel, CH 6 84
Mason, Derek Basel, CH 4 21
Parola, Cristina Basel, CH 2 1
Pogson, Mark North Bend, US 8 2
Reddy, Sai Basel, CH 9 105

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