Pig genome-wide specific sgRNA library, preparation method therefor and application thereof

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United States of America Patent

PATENT NO 11898270
APP PUB NO 20200325597A1
SERIAL NO

16628481

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Provided is a pig genome-wide specific sgRNA library, a preparation method therefor, and an application thereof. The sgRNA is targeted at a pig genome-wide protein-coding gene, lincRNA and/or miRNA. Specifically, an sgRNA construct has the following structure: AL-N20-AR, wherein AL is the left homology arm sequence located at the upstream of the coding sequence of a pig specific SgRNA, N20 is the coding sequence of the pig specific SgRNA, and AR is a right homology arm sequence located at the downstream of the coding sequence of the pig specific sgRNA. The sgRNA library can be used for screening functional genes of a pig or for preparing a kit.

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Patent Owner(s)

Patent OwnerAddress
HUAZHONG ARGICULTURAL UNIVERSITYNO 1 SHIZISHAN STREET HUAZHONG ARGICULTURAL UNIVERSITY HONGSHAN DISTRICT WUHAN HUBEI 430070

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Chen, Yilong Hubei, CN 68 167
Du, Xiaoyong Hubei, CN 8 18
Gao, Yang Hubei, CN 446 6138
Han, Xiaosong Hubei, CN 1 0
Li, Xinyun Hubei, CN 5 2
Liu, Xiangdong Hubei, CN 50 467
Liu, Xiaolei Hubei, CN 115 570
Ma, Yunlong Hubei, CN 11 0
Miao, Yiliang Hubei, CN 1 0
Xie, Shengsong Hubei, CN 3 0
Yang, Gaojuan Hubei, CN 1 0
Zhao, Changzhi Hubei, CN 1 0
Zhao, Shuhong Hubei, CN 4 0

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