Library preparation methods and compositions and uses therefor

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United States of America Patent

PATENT NO 12065643
APP PUB NO 20220315917A1
SERIAL NO

17302674

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Abstract

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Provided are methods for preparing a library of target nucleic acid sequences, as well as compositions and uses therefor. Methods comprise contacting a nucleic acid sample with a plurality of adaptors capable of amplification of one or more target nucleic acid sequences under conditions wherein the target nucleic acid(s) undergo a first amplification; digesting the resulting first amplification products; repairing the digested target amplicons; and amplifying the repaired products in a second amplification, thereby producing a library of target nucleic acid sequence. Each of the plurality of adaptor compositions comprise a handle and a targeted nucleic acid sequence and optionally one or more tag sequences. Provided methods may be carried out in a single, addition only workflow reaction, allowing for rapid production of highly multiplexed targeted libraries, optionally including unique tag sequences. Resulting library compositions are useful for a variety of applications, including sequencing applications.

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Patent Owner(s)

Patent OwnerAddress
LIFE TECHNOLOGIES CORPORATION5823 NEWTON DRIVE CARLSBAD CA 92008

International Classification(s)

Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Andersen, Mark Carlsbad, US 66 1175
Chen, Sihong Vista, US 28 23
Luo, Guobin Oceanside, US 31 459
Mazur, Daniel San Diego, US 45 256
Peng, Xinzhan San Diego, US 20 481

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