DNA sequencing method

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United States of America

PATENT NO 12129520
APP PUB NO 20190330695A1
SERIAL NO

16327535

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Abstract

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Disclosed is a DNA sequencing method. The DNA sequencing method of this invention comprises: (1) adding a tag sequence at the 3′ terminus of the DNA to be sequenced so as to form a DNA to be sequenced including the tag sequence, the nucleotide sequence of said tag sequence being the reverse complement of the nucleotide sequence of the sequencing primer; (2) mixing the DNA to be sequenced including the tag sequence and the sequencing primer so as to form a product having a 5′ terminus double strand and a single main strand; (3) after step (2) is completed, the product is mixed separately with dATP, dCTP, dTTP, and dGTP to obtain four systems, each system is separately added to a single-molecule device modified by a DNA polymerase, and electric signals are read. Experiments verify that the method of this invention performs DNA sequencing and has important application value.

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Patent Owner(s)

Patent OwnerAddress
PEKING UNIVERSITY100871 NO 5 THE SUMMER PALACE ROAD BEIJING HAIDIAN DISTRICT BEIJING CITY BEIJING CITY 100871

International Classification(s)

Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Ci, Haina Beijing, CN 1 3
Guo, Xuefeng Beijing, CN 12 65
Wang, Xiaolong Beijing, CN 101 479

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