Charge perturbation detection system for DNA and other molecules

Number of patents in Portfolio can not be more than 2000

United States of America

PATENT NO 12139749
APP PUB NO 20210230678A1
SERIAL NO

17087336

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Abstract

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Methods and apparatus for direct detection of chemical reactions are provided. Electric charge perturbations of the local environment during enzyme-catalyzed reactions are sensed by an electrode system with an immobilized target molecule. The charge perturbation caused by the polymerase reaction can uniquely identify a DNA sequence. The polymerization process generates local perturbations of charge in the solution near the electrode surface and induces a charge in a polarazible gold electrode. This event is detected as a transient current by a voltage clamp amplifier. Detection of single nucleotides in a sequence can be determined by dispensing individual dNTPs to the electrode solution and detecting the charge perturbations. Alternatively, multiple bases can be determined at the same time using a mix of all dNTPs with subsequent analysis of the resulting signal. This technique may be adapted to other reaction determinations, such as enzymatic reactions, other electrode configurations, and other amplifying circuits.

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Patent Owner(s)

Patent OwnerAddress
THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITYOFFICE OF THE GENERAL COUNSEL BUILDING 170 THIRD FLOOR MAIN QUAD P O BOX 20386 STANFORD CA 94305-2038

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Davis, Ronald W Stanford, US 96 3007
Karhanek, Miloslav Santa Cruz, US 15 400
Pourmand, Nader San Mateo, US 39 1094

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