Sequence tag directed subassembly of short sequencing reads into long sequencing reads

Number of patents in Portfolio can not be more than 2000

United States of America

PATENT NO 12152236
APP PUB NO 20230348896A1
SERIAL NO

18050382

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Abstract

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The present disclosure relates to a method for preparing a DNA sequencing library that brings more distal fragmentation breakpoints into close proximity to the non-degenerate sequence tag. In an embodiment, the method comprises circularizing a target fragment library with a plurality of adaptor molecules to produce a population of first circularized double-stranded DNA molecules, wherein the plurality of adaptor molecules comprises a first defined sequence P1 comprising a first restriction enzyme recognition site R1, a degenerate sequence tag, and a second defined sequence P2 comprising a second restriction enzyme recognition site R2, such that at least one of the first circularized double-stranded DNA molecule comprises a non-degenerate sequence tag and a member of the target fragment library. The method is useful because some sequencing platforms perform optimally with template molecules that are relatively short, for example, less than about 500 base pairs in length.

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Patent Owner(s)

Patent OwnerAddress
UNIVERSITY OF WASHINGTON4545 ROOSEVELT WAY NE SUITE 400 SEATTLE WA 98105

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Hiatt, Joseph Seattle, US 10 966
Patwardhan, Rupali Seattle, US 10 966
Shendure, Jay Seattle, US 35 2522
Turner, Emily Seattle, US 9 932

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