Apparatus and methods for fluorescence imaging using radiofrequency-multiplexed excitation

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United States of America

PATENT NO 12158421
APP PUB NO 20230052995A1
SERIAL NO

17745206

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Abstract

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Apparatus and methods for fluorescence imaging using radiofrequency multiplexed excitation. One apparatus splits an excitation laser beam into two arms of a Mach-Zehnder interferometer. The light in the first beam is frequency shifted by an acousto-optic deflector, which is driven by a phase-engineered radiofrequency comb designed to minimize peak-to-average power ratio. This RF comb generates multiple deflected optical beams possessing a range of output angles and frequency shifts. The second beam is shifted in frequency using an acousto-optic frequency shifter. After combining at a second beam splitter, the two beams are focused to a line on the sample using a conventional laser scanning microscope lens system. The acousto-optic deflectors frequency-encode the simultaneous excitation of an entire row of pixels, which enables detection and de-multiplexing of fluorescence images using a single photomultiplier tube and digital phase-coherent signal recovery techniques.

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THE REGENTS OF THE UNIVERSITY OF CALIFORNIA1111 FRANKLIN STREET 5TH FLOOR OAKLAND CA 94607-5200

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Buckley, Brandon Los Angeles, US 24 321
Diebold, Eric D Los Angeles, US 43 189
Jalali, Bahram Los Angeles, US 73 723

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