Simplified method for size-purification of small oligonucleotides via gel electrophoresis

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United States of America

PATENT NO 12188009
APP PUB NO 20190352633A1
SERIAL NO

16469242

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Abstract

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Methods for simplified, rapid gel electrophoresis size-purification and downstream sequencing of an Oligo Library, for example an miRNA Library, based on co-purifying a DNA ladder molecular weight standard customized to co-migrate with Library to provide highly salient bands on the gel at the upper and lower ends of the Library size range, permitting precise cutting and avoiding the negative effects of gel distortion, variation of DNA migration rate in different lanes, and the difficulty of matching sample to ladder across a large distance. The methods may be applied to increase yield and purity in an Oligo Library of low-quality or low input Oligo, and in particular in small RNA samples.

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Patent OwnerAddress
UNIVERSITY OF CINCINNATI2900 READING ROAD SUITE 260 CINCINNATI OH 45206

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Ho, Shuk-Mei Cincinnati, US 5 1
Zhang, Xiang Cincinnati, US 387 2583

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