Importance

Loading Importance Indicators...

Abstract

A hormone-free culture method for a citrus shoot tip is provided according to the following steps: (1) selecting a citrus branch to make an explant; (2) washing with sterile water after disinfection; (3) inserting the explant into a Murashige and Skoog (MS) solid medium and culturing to produce a branch with new buds; (4) preparing a Murashge and Tucker (MT) liquid medium solution, where a mass concentration of sucrose is 7-8%; (5) removing an outer leave of the new bud, cutting and taking a shoot tip, and inoculating in a liquid medium for primary culture; (6) selecting a seed of a trifoliate orange or a citrange and peeling off a testa; washing with the sterile water after disinfection; (7) inoculating a resulting seed into a test tube containing the MS solid medium and culturing in dark to obtain an etiolated seedling; (8) cutting a T-shaped incision and placing the shoot tip in a middle of the T-shaped incision to obtain a grafted seedling; and (9) grafting culture, and then conducting a secondary grafting in a greenhouse when a scion bud grows to 0.5-1 cm. According to the present invention, regenerated seedlings are obtained without using hormones, and the efficiency of plant shoot tip culture is significantly improved, which makes the method of the present invention widely used in different citrus varieties.

Loading the Abstract Image...

First Claim