from embryonic tip tissue

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United States of America Patent

PATENT NO 12201076
APP PUB NO 20240284846A1
SERIAL NO

18651494

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Abstract

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A method for obtaining regenerated seedlings of Brassica campestris L. ssp. chinensis from embryonic tip tissues, including the following steps. A seed is inoculated to a germination medium for dark culture for 60 h. The testa, root tip, two cotyledons and middle growing point of the resultant germinated seed are removed, and an embryonic tip with a length of 3-5 mm is retained as an explant. The explant is sequentially subjected to low-temperature pre-culture in a pre-culture medium for 36 h, room-temperature shaking culture in a liquid bud induction culture medium for 10 min, and bud induction culture in a bud induction culture medium for 20 d. A regenerated plant with 5-6 leaves is transferred to a rooting medium for rooting culture for about 2 weeks, and a well-rooted plant is collected, and subjected to hardening and transplantation into a filed.

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Patent Owner(s)

Patent OwnerAddress
ANHUI AGRICULTURAL UNIVERSITY230001 NO 130 CHANGJIANG WEST ROAD HEFEI ANHUI HEFEI CITY ANHUI PROVINCE 230001
YINGSHANG SHILI ECOLOGICAL AGRICULTURE TECHNOLOGY CO LTDDING ZHUANG SHILI VILLAGE NANZHAO TOWN YINGSHANG COUNTY FUYANG

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Chen, Guohu Hefei, CN 1 0
Li, Ting Hefei, CN 218 986
Liu, Xueqing Hefei, CN 5 2
Tang, Xiaoyan Hefei, CN 23 15
Wang, Chenggang Hefei, CN 5 0
Wang, Ying Hefei, CN 968 7243
Wen, Hongwei Hefei, CN 2 0
Wu, Siwen Hefei, CN 1 0
Yin, Qian Hefei, CN 26 43

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