N- and C-terminal tandem tag system for purification

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United States of America

PATENT NO 12209139
APP PUB NO 20220056072A1
SERIAL NO

17408328

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Abstract

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Expression vectors and methods of protein purification, which allow for selection of full length protein over truncated forms of the protein being purified, are disclosed. The methods express a target protein as a three domain fusion, represented by formula I:A-[Li]-B-[L2]-C, where A is a first purification tag domain, C is the second purification tag domain and B is the target protein domain. A, B and C are preferably covalently linked by linkers, L1 and L2 may be optional. The purification tags at the N and C termini are different. The purification tags at the N and C termini are different. Expression vectors including nucleic acid sequences which encode the fusion protein represented by formula I are also disclosed. The vectors are used with host expression systems such as insect, yeast, or mammalian cells to express the target protein, which is subsequently purified as a function of the different affinity tags.

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Patent Owner(s)

Patent OwnerAddress
KING ABDULLAH UNIVERSITY OF SCIENCE AND TECHNOLOGYTHUWAL 23955

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Hamdan, Samir M Thuwal, SA 7 0
Raducanu, Vlad-Stefan Thuwal, SA 4 0

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