Methods for determining spatial and temporal gene expression dynamics during adult neurogenesis in single cells

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United States of America

PATENT NO 12221720
APP PUB NO 20210395821A1
SERIAL NO

16763681

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Abstract

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Techniques Nuc-seq, Div-Seq, and Dronc-Seq are allow for unbiased analysis of any complex tissue. Nuc-Seq, a scalable single nucleus RNA-Seq method, can sensitively identify closely related cell types, including within the adult hippocampus. Div-seq combines Nuc-Seq with EdU-mediated labeling of proliferating cells, allowing tracking of transcriptional dynamics of newborn neurons in an adult neurogenic region in the hippocampus. Dronc-Seq uses a microfluidic device to co-encapsulate individual nuclei in reverse emulsion aqueous droplets in an oil medium together with one uniquely barcoded mRNA-capture bead.

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Patent Owner(s)

  • THE BROAD INSTITUTE, INC.; MASSACHUSETTS INSTITUTE OF TECHNOLOGY; PRESIDENT AND FELLOWS OF HARVARD COLLEGE

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Avraham-Davidi, Inbal Cambridge, US 2 1
Basu, Anindita Cambridge, US 7 362
Drokhlyansky, Eugene Cambridge, US 4 7
Habib, Naomi Cambridge, US 6 495
Regev, Aviv Cambridge, US 146 1011
Rozenblatt-Rosen, Orit Cambridge, US 20 117
Weitz, David A Cambridge, US 200 11534

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