Compositions and methods of labeling mitochondrial nucleic acids and sequencing and analysis thereof

Number of patents in Portfolio can not be more than 2000

United States of America

PATENT NO 12258615
APP PUB NO 20220056502A1
SERIAL NO

17409731

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Abstract

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Methods of labeling, amplifying, and sequencing mitochondrial DNA are provided. The labeling methods typically include using polymerase-based extension of one or more unique molecular identifier (UMI) primers along a target mitochondrial DNA template, each UMI primer including a universal primer sequence, a unique molecular identifier sequence, and a mitochondrial DNA binding sequence. Amplification of the labeled-target mitochondrial DNA can include, for example, polymerase chain reaction (e.g., high-fidelity long-range). Methods of determining the sequence of labeled-target mitochondrial DNA can include use of a long-read sequencing platform. The methods may further including one or more of grouping sequences having the same UMI into one of more groups, determining the sequence of each labeled-target mitochondrial DNA by determining the consensus sequence of each group, and identifying polymorphisms in one or more of the labeled-target mitochondrial DNA. The methods can be used in, for example, preimplantation genetic testing.

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Patent Owner(s)

Patent OwnerAddress
KING ABDULLAH UNIVERSITY OF SCIENCE AND TECHNOLOGY4700 KING ABDULLAH UNIVERSITY OF SCIENCE AND TECHNOLOGY THUWAL-JEDDAH 23955

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Bi, Chongwei Thuwal, SA 4 1
Li, Mo Thuwal, SA 50 318

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