Tumor cells having tumorigenic potential but lacking invasive/metastatic potential, method for preparing them and screening method for metastasis-related genes using the same
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United States of America Patent
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Jul 27, 2004
Grant Date -
Jul 5, 2001
app pub date -
Aug 28, 1998
filing date -
Feb 29, 1996
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Expired
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Abstract
Tumor cells having tumorigenic potential but lacking invasive/metastatic potential are established by introducing an oncogene of the ras family into a BALB/c 3T3 A31-variant cell. A screening method for genes having the property of conferring invasive/metastatic potential is also provided, which comprises transfecting DNA derived from a tumor tissue obtained from the surface or inside of a mammal or derived from a tumor cell line into tumor cells having tumorigenic potential but lacking invasive/metastatic potential, isolating cells having acquired invasive/metastatic potential and extracting DNA therefrom.
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