Labeled protein and its producing method, labeling compound to be used in the method, and method for analyzing function of genes
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United States of America Patent
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May 9, 2006
Grant Date -
Jul 12, 2001
app pub date -
Feb 28, 2001
filing date -
May 15, 1998
priority date (Note) -
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Abstract
A protein having a labeling compound attached to its C-terminal, in which the compound comprises a label portion comprising a label substance and an acceptor portion comprising a compound having an ability of binding to a C-terminal of a synthesized protein when protein synthesis is carried out in a cell-free protein synthesis system or in a living cell is provided. Also, a method for producing the protein comprising the step of performing synthesis of a protein in a cell-free protein synthesis system or in a living cell in the presence of a labeling compound comprising a label portion comprising a label substance and an acceptor portion comprising a compound having an ability of binding to a C-terminal of a synthesized protein when protein synthesis is carried out in the cell-free protein synthesis system or in the living cell, the labeling compound being present at a concentration effective for the labeling compound to bind to the C-terminal of the synthesized protein is provided. Also, the labeling compound and a method for analyzing a function of a gene are further provided.
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