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United States of America Patent
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N/A
Issued Date -
Aug 2, 2001
app pub date -
Mar 26, 1999
filing date -
Mar 26, 1999
priority date (Note) -
Abandoned
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Abstract
A method for the high level production of active, properly processed recombinant protein in trans-splicing organisms is disclosed. The method involves the integration of the gene encoding the recombinant protein of interest into a chromosomal locus where it is transcribed under the direction of the rRNA promoter. The gene is also operably linked to intergenic regions allowing the protein to be translated in these organisms. The recombinant organisms expressing a therapeutic protein can also be used to treat a disease or undesirable condition which is characterized by a deficiency in that protein.
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