Nipple aspirate fluid specific microarrays
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United States of America Patent
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N/A
Issued Date -
Nov 8, 2001
app pub date -
Jan 19, 2001
filing date -
Jan 21, 2000
priority date (Note) -
Abandoned
status (Latency Note)
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Abstract
A method is provided for aid in the diagnosis of breast cancer. Nipple aspirate fluid ('NAF') is collected from the breast via a modified breast pump device. Breast epithelial cells ('BECs') and proteins present in NAF are isolated. Nucleic acid is extracted from the BECs. The nucleic acids and secreted proteins are analyzed via a low-density nucleic acid and peptidic biopolymeric array, respectively. The level of RNA transcription, protein expression, and heterozygosity is compared to that of normal control cells. This invention has particular application to the early detection of breast cancer and the study of individual responses to chemo- and radiotherapeutic exposures.
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