Monoclonal antibodies to 4,4'-dinitrocarbanilide and a method for analyzing for the drug nicarbazin
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United States of America Patent
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Oct 15, 2002
Grant Date -
Nov 8, 2001
app pub date -
Nov 29, 2000
filing date -
Nov 30, 1999
priority date (Note) -
Expired
status (Latency Note)
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Abstract
Hybridoma cell lines have been generated which produce and secrete monoclonal antibodies which selectively bind to 4,4'-dinitrocarbanilide (DNC), the active agent of nicarbazin. These hybridomas may be obtained by using as an immunization agent or immunogen, p-nitroaniline which has been conjugated to an immunogenic carrier. DNC in biological samples may be detected and quantified by contacting the sample with the antibodies to form a DNC/antibody immunocomplex when DNC is present, which immunocomplex may then be detected. The monoclonal antibodies also may be incorporated into kits for the detection and quantification of DNC and/or nicarbazin.
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