Sex-chromosome-specific proteins, species specific and sperm specific proteins and methods for their identification and isolation
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United States of America Patent
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Issued Date -
Nov 15, 2001
app pub date -
Dec 7, 2000
filing date -
Dec 7, 1999
priority date (Note) -
Abandoned
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Abstract
Sex-chromosome-specific molecules associated with animal sperm cell membranes, and the method for isolating them, are described. An X chromosome specific molecule, a protein, having an apparent molecular weight of 32 kDa on SDS-PAGE is specifically described. The method involves preparing a cell membrane fraction from animal sperm cells; treating the cell membrane fraction with one or more substances which bind to X or Y chromosome specific molecules in the cell membrane fraction to form conjugates between the X or Y chromosome specific molecules and the substances; separating the material in the cell membrane fraction which does not bind to the substances to obtain a subfraction containing sex-chromosome-specific molecules. Methods of using the sex-chromosome-specific molecules for sperm sexing are also described.
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Patent Owner(s)
Patent Owner | Address | |
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UNIVERSITY OF GUELPH | RESEARCH OFFICE AXELROD INSTITUTE OF ICHTHYOLOGY (BUILDING 92) 50 STONE RD EAST GUELPH N1G 2W1 |
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Inventor(s)
Inventor Name | Address | # of filed Patents | Total Citations |
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Blecher, Stan | Guelph, CA | 2 | 1 |
Detmar, Jacqueline | Cambridge, CA | 2 | 1 |
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Fee | Large entity fee | small entity fee | micro entity fee |
---|---|---|---|
Surcharge after expiration - Late payment is unavoidable | $700.00 | $350.00 | $175.00 |
Surcharge after expiration - Late payment is unintentional | $1,640.00 | $820.00 | $410.00 |
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