Parthenogenic oocyte activation
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United States of America Patent
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N/A
Issued Date -
Nov 29, 2001
app pub date -
Feb 27, 2001
filing date -
Mar 4, 1996
priority date (Note) -
Abandoned
status (Latency Note)
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Abstract
A process of parthenogenic activation of mammalian oocytes which includes increasing intercellular levels of divalent cations in the oocyte; and reducing phosphorylation of cellular proteins in the oocyte. One method of accomplishing this is by introducing Ca.sup.2+ free cation, such as inomycin, to the oocyte and then preventing phosphorylation of the cellular proteins within the oocyte by adding a serine-threonine kinase inhibitor, such as 6-dimethylaminopurine (DMAP).
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