Methods for producing nucleic acid hybridization probes that amplify hybridization signal by promoting network formation

Number of patents in Portfolio can not be more than 2000

United States of America Patent

PATENT NO 7763421
APP PUB NO 20010051342A1
SERIAL NO

09874849

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ATTORNEY / AGENT: (SPONSORED)

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Abstract

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This invention describes methods for generating nucleic acid probes that improve the sensitivity of hybridization assays. The sensitivity increase results from structural modifications of nucleic acids that promote network formation during hybridization with the result that a single target molecule becomes attached to a complex of many probe molecules. The structural modification involves fragmentation of the probe nucleic acid followed by joining the fragments together such that their order and orientation and number is altered from the original probe molecule. The result is the generation of permuted probe libraries. Individual members of permuted probe libraries can be isolated, amplified and perpetuated. Libraries can be prepared with additional sequences not present in the target and the fraction of the library made up by such sequences controlled. Probes for different targets can incorporate different non-target sequences in hyper-molar quantities permitting sensitive detection of multiple hybridization targets in the same sample.

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Patent Owner(s)

Patent OwnerAddressTotal Patents
VENTANA MEDICAL SYSTEMS, INC.TUCSON, AZ188

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Farrell, Michael Patrick Sugar Grove, US 3 27

Cited Art Landscape

Patent Info (Count) # Cites Year
 
SIEMENS HEALTHCARE DIAGNOSTICS INC. (2)
5124246 Nucleic acid multimers and amplified nucleic acid hybridization assays using same 385 1989
* 5849481 Nucleic acid hybridization assays employing large comb-type branched polynucleotides 80 1995
* Cited By Examiner

Patent Citation Ranking

Forward Cite Landscape

Patent Info (Count) # Cites Year
 
VENTANA MEDICAL SYSTEMS, INC. (2)
* 8148075 Methods for producing nucleic acid hybridization probes that amplify hybridization signal by promoting network formation 0 2010
* 2011/0160,076 Methods for producing uniquely specific nucleic acid probes 2 2010
* Cited By Examiner

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