Nucleic acids encoding cold sensitive mutant DNA polymerases
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United States of America Patent
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Nov 13, 2001
Grant Date -
Jan 10, 2002
app pub date -
Feb 6, 2001
filing date -
Jun 6, 2000
priority date (Note) -
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Abstract
Provided are mutant DNA polymerases having at least one mutation which exhibit substantially reduced polymerase activity at 25.degree. C. when compared to the same DNA polymerases without the at least one mutation and which exhibit normal or near-normal polymerase activity at optimum temperatures when compared to the same DNA polymerases without the at least one mutation. Also provided are amino acid sequences and nucleic acid sequences encoding such DNA polymerases, and vector plasmids and host cells suitable for the expression of these sequences. Also described herein are improved methods for performing polymerase chain reaction (PCR) amplification and other genetic manipulations and analyses using the mutant DNA polymerases of the invention.
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