Method and apparatus for intracellular electro-manipulation
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United States of America Patent
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Issued Date -
Jan 24, 2002
app pub date -
Feb 7, 2001
filing date -
Aug 4, 1999
priority date (Note) -
Abandoned
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Abstract
A method for intracellular electro-manipulation is provided. The method includes applying one or more ultrashort electric field pulse to target cells in a tissue. The ultrashort electric field pulses have sufficient amplitude and duration to modify subcellular structures in the target cells and do not exceed the breakdown field of the medium containing the target cells. The ultrashort electric field pulses can be used to treat a neoplastic condition in a patient by applying one or more ultrashort electric field pulses to at least a portion of a neoplasm in vivo. Such treatments typically involve the application of electric field pulses which have a pulse duration of no more than 1 microsecond and an amplitude of at least 10 kV/cm. An apparatus for destroying target cells in vivo is also provided. The apparatus includes a pulse generator capable of producing one or more ultrashort electric pulse outputs and a delivery system capable of directing the electric pulse output to target cells in vivo.
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