High temperature reverse transcription using mutant DNA polymerases

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United States of America Patent

PATENT NO 7179590
SERIAL NO

09823649

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Abstract

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The present invention relates to improved reverse transcription methods using a modified thermostable DNA polymerases, particularly in a magnesium ion buffer. These methods are particularly useful in combined reverse-transcription/amplification reactions.

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Patent Owner(s)

Patent OwnerAddress
ROCHE MOLECULAR SYSTEMS INC4300 HACIENDA DRIVE PLEASANTON CA 94588

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Elfstrom, Carita Maria San Francisco, CA 1 12
Gelfand, David Harrow Oakland, CA 16 294
Higuchi, Russell Gene Alameda, CA 4 231
Myers, Thomas William Alameda, CA 2 19
Schonbrunner, Nancy Jeneane Moraga, CA 1 12
Smith, Edward Soh San Francisco, CA 1 12
Wang, Alice Ming Lafayette, CA 2 33

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