METHOD FOR IDENTIFYING SEX SPECIFIC AND SPECIES SPECIFIC MOLECULES, MOLECULES IDENTIFIED USING THE METHOD, AND USES OF THE MOLECULES
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United States of America Patent
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app pub date -
Aug 17, 1998
filing date -
Aug 9, 1996
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Abandoned
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Abstract
A met for identifying non-sex specific and sex specific molecules associated with animal cell membranes is described. The method involves preparing a cell membrane fraction from adult, fetal, or embryonic animal cells; treating the cell membrane fraction with one or more substances which bind to non-sex specific molecules in the cell membrane fraction to form conjugates between the non-sex specific molecules and the substances; separating the material in the cell membrane fraction which does not bind to the substances to obtain a subfraction containing sex specific molecules; optionally, removing the substances in the conjugates to obtain a subfraction containing non-sex specific molecules; and, isolating the sex specific molecules and optionally none specific molecules in the subfractions. Sex specific and non-sex specific molecules and methods of using the sex specific and non-sex specific molecules for sexing are also described.
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