1-aminocyclopropane-1-carboxylate synthase gene from Rosa to control ethylene levels in roses
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United States of America Patent
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Issued Date -
Jun 27, 2002
app pub date -
Feb 2, 2001
filing date -
Oct 1, 1996
priority date (Note) -
Abandoned
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Abstract
A gene which encodes an ACC synthase is identified for the rose plants, specifically Rosa (cardinal red). This gene is shown as modified to achieve a transgenic plant which resists wilting and the like as a result of reduced ethylene production. This alteration is reproduced by the transformed plant. Isolation of high quality mRNA is achieved through use and adaptation of a 2-butoxyethanol precipitation technique using large amount of initial tissue in order to achieve critical mass for precipitation.
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