Fluorogenic compounds and uses therefor
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United States of America Patent
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May 20, 2003
Grant Date -
Oct 10, 2002
app pub date -
Mar 11, 2002
filing date -
Jul 7, 2000
priority date (Note) -
Expired
status (Latency Note)
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Abstract
Disclosed are novel coumarin based fluorogenic compounds useful in assaying for biological activity. Specifically, these fluorogenic compounds exhibit fluorescence at particular wavelengths when cleaved by target enzymes. Preferred compounds include sugar and peptide derivatives of umbelliferone derivatives bearing a heterocyclic five membered ring at the 3-position. These compounds can be used for rapidly detecting food pathogens and for determining sterilization effectiveness. The compounds may also be used in a form bounded to a polymeric support or to a biomolecule or macromolecule.
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