Compositions and methods for isolating genes comprising subcellular localization sequences
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United States of America Patent
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Issued Date -
Oct 17, 2002
app pub date -
Mar 26, 2002
filing date -
Mar 27, 2001
priority date (Note) -
Abandoned
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Abstract
The present invention provides an expression vector and library thereof suited for categorizing and identifying genes comprising subcellular localization sequences. The invention vectors are particularly suited for isolating extracellular membrane bound, extracellular or secreted proteins. The present invention also provides kits and eukaryotic host cells comprising the invention vectors. Further provided by the invention are methods of using the subject vectors for cloning genes encoding proteins that are preferentially located in certain subcellular locations. Also included is a method of determining the subcellular location of a protein.
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