Method and apparatus for culturing cells
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United States of America Patent
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Issued Date -
Oct 24, 2002
app pub date -
Mar 27, 2002
filing date -
Mar 27, 2001
priority date (Note) -
Abandoned
status (Latency Note)
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Abstract
A method and apparatus for improving the efficiency of the growth of cell and tissue cultures in vitro is disclosed. The improvement in efficiency is achieved through the use of a scaffold formed from an open cell polymer foam that has been surface treated by an oxidative plasma discharge. In one embodiment, the polymer foam is a polystyrene foam treated with an oxygen gas plasma to functionalize the surface of the polymer. In the preferred embodiment, the scaffold is used as an insert for a bioreactor to culture cells and tissue. The scaffold is formed from a porous polymer structure having a continuous polymer matrix with a plurality of interconnected open pores. The pore size typically ranges from about 50 microns to about 500 microns.
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