High-throughput nucleic acid polymerase devices and methods for their use
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United States of America Patent
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Issued Date -
Nov 21, 2002
app pub date -
Mar 27, 2001
filing date -
Mar 27, 2001
priority date (Note) -
Abandoned
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Abstract
High throughput lyophilized polymerase devices and methods for their use in the production of nucleic acids using template dependent polymerase reactions are provided. The subject devices are typically made up of a multi-well substrate that includes in a least one well a lyophilized nucleic acid polymerase composition. The subject nucleic acid polymerase compositions include at least one polymerase and a carbohydrate stabilizing composition that is made up of at least one low molecular weight sugar and a starch. In many embodiments, the compositions also include buffer components and nucleotides, as well as a temperature dependent polymerase inhibitor, e.g., a polymerase specific antibody. Also provided are kits that include the subject devices.
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