Centrifuge apparatus and methods for separating components from a cell culture device
Number of patents in Portfolio can not be more than 2000
United States of America Patent
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Issued Date -
Nov 21, 2002
app pub date -
May 21, 2001
filing date -
May 21, 2001
priority date (Note) -
Abandoned
status (Latency Note)
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Abstract
An apparatus for use in a swinging bucket centrifuge rotor, the apparatus comprising a pair of sidewalls wherein each sidewall comprises a plurality of longitudinally spaced and laterally disposed partitions; wherein each pair of adjacent partitions defines a slot which is dimensioned to receive a cell culture device. The apparatus comprises a centrifuge bucket or an adaptor for use with a centrifuge bucket. Also provided is a method for harvesting one or more components of a cell culture contained within a cell culture device by centrifuging the cell culture device as supported by the apparatus during centrifugation, and then removing separately the one or more components of the cell culture from the centrifuged cell culture device. Further provided is a method for harvesting anchorage-dependent cells by distending the membrane surface to which the cells adhere in a process of loosening the cells, contacting and mixing the loosened cells with a fluid in forming a cell suspension, and then harvesting the anchorage-dependent cells by removing the cell suspension from the cell culture device.
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