Methods for analyzing nucleic acids using a type IIs restriction endonuclease
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United States of America Patent
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Jan 21, 2003
Issued Date -
N/A
app pub date -
Aug 27, 2001
filing date -
Sep 16, 1994
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Expired
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Abstract
The present invention relates to novel methods for sequencing and mapping genetic markers in polynucleotide sequences using Type-IIs restriction endonucleases. The methods herein described result in the 'capturing' and determination of specific oligonucleotide sequences located adjacent to Type-IIs restriction sites. The resulting sequences are useful as effective markers for use in genetic mapping, screening and manipulation.
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