Yeast cells engineered to produce pheromone system protein surrogates, and uses therefor
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United States of America Patent
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app pub date -
May 10, 1999
filing date -
Mar 31, 1993
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Abstract
Yeast cells are engineered to express both a surrogate of a pheromone system protein (e.g., enzymes involved in maturation of .alpha.-factor, transporters of a-factor, pheromone receptors, etc.) and a potential peptide modulator of the surrogate, in such a manner that the inhibition or activation of the surrogate affects a screenable or selectable trait of the yeast cells. Various additional features improve the signal-to-noise ratio of the screening/selection system.
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