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Abstract

The present invention provides a fast, simple and reliable polymerase thermocycling reaction procedure for the linear amplification of nucleic acid sequences from cellular RNAs or genomes or both. The principle of this polymerase thermocycling reaction method relies upon the thermal cycling steps of promoter-linked nucleic acid template synthesis and in-vitro transcriptional amplification to bring up the amount of desired nucleic acid sequences up to two thousand fold within one cycle of the above procedure. Neither RNase H activity nor alkaline degradation is used in order to protect the RNA sequences of resulting amplified products which are ready for further applications, such as genechip/microarray analysis, in-vitro translation reaction and induction of RNA interference. Without the preferential amplification drawback of PCR/RT-PCR methods, the accuracy and resolution of current molecular biology analysis and diagnosis can be significantly improved by the present invention.

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First Claim